Regulatory

Part:BBa_K3249000:Design

Designed by: Omar El-Fahmawi   Group: iGEM19_UC_Davis   (2019-10-04)


CIBN-dCas9-CIBN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1360
    Illegal PstI site found at 2782
    Illegal PstI site found at 2986
    Illegal PstI site found at 3016
    Illegal PstI site found at 4228
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4717
    Illegal PstI site found at 1360
    Illegal PstI site found at 2782
    Illegal PstI site found at 2986
    Illegal PstI site found at 3016
    Illegal PstI site found at 4228
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 821
    Illegal BamHI site found at 4708
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1360
    Illegal PstI site found at 2782
    Illegal PstI site found at 2986
    Illegal PstI site found at 3016
    Illegal PstI site found at 4228
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1360
    Illegal PstI site found at 2782
    Illegal PstI site found at 2986
    Illegal PstI site found at 3016
    Illegal PstI site found at 4228
    Illegal NgoMIV site found at 109
    Illegal NgoMIV site found at 1648
    Illegal NgoMIV site found at 2752
    Illegal NgoMIV site found at 2825
    Illegal NgoMIV site found at 3310
    Illegal NgoMIV site found at 4219
    Illegal NgoMIV site found at 4867
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Adding an additional CIBN region to the dCas9 allows for increased transcriptional activation because it provides another place for CRY2 to bind to and activate the system.


Source

The CIBN regions come from the organism Arabidopsis thaliana. The dCas9 originates from the Streptococcus pyogenes genome.

References